The #93 got back in the saddle for FP1 opening session and ended in 15th place, setting a fastest lap of 1:47.419. That left the returning 33-year-old 1.177s off the pace set by session leader and Catalan GP winner, Fabio Di Giannantonio (Pertamina Enduro VR46 Racing Team).
Following FP1, Marquez visited the medical centre for a scheduled check-up to assess his condition after returning from injury. After that review, the Ducati rider was declared fit to compete across the remainder of the weekend at Mugello, aiming to put on a show for his team's home event in front of the Ducatisti.